3D-STED Microscopy to Dissect the Supra-Molecular Structure of Cell Junctions

October 2nd, 2017 ALF HONIGMANN MPI of Molecular Cell Biology and Genetics

My lab is focused on revealing the structure, the dynamics and the functions of our cell membranes. To this end we use super-resolution STED-microscopy to resolve membrane complexes with a close to molecular resolution. We combine STED with fluorescence correlation spectroscopy (STED-FCS) to capture fast diffusion dynamics of lipids and proteins in the membrane. And we use in vitro membrane reconstitutions to compare live cell data to simple model systems. In this presentation I will give an overview about the methods and show our latest results on the supra-molecular structure of cell adhesion junctions.

Seminar, October 2, 2017, 12:00. ICFO Seminar Room

Hosted by Maria García-Parajo