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3D super resolution fluorescence imaging of neurons with STORM facilitates neuronal tracing and connectional mapping.

Advanced Fluorescence Imaging and Biophysics

Prof. Dr. Melike Lakadamyali

    Activities:

The Advanced Fluorescence Imaging and Biophysics group is an interdisciplinary group that works at the interface of advance light microscopy techniques and their biological applications. We are interested in developing fluorescence imaging techniques with high spatiotemporal resolution and use these techniques to study questions in virology, neuroscience and other fields. Our aim is to elucidate fundamental biological questions that also have important implications for health. We are a newly established group (starting date September 2010) and expect to rapidly grow into a multidisciplinary research team of physicists, engineers and biologists.

    Available Resources
  • Epifluorescence microscope for live cell imaging and single particle tracking
  • TIRF microscope for super resolution imaging with STORM and PALM

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Advanced Fluorescence Imaging and Biophysics

Prof. Dr. Melike Lakadamyali

Advanced Fluorescence Imaging and Biophysics

Prof. Dr. Melike Lakadamyali

    Invited Talks
  • Probing cargo transport with correlated live cell and super-resolution microscopy M. Lakadamyali EMBL Advanced Course, Advanced Imaging Techniques, Heidelberg, Germany (2012)
  • Super-resolution microscopy and its biological applications M. Lakadamyali CNIO, Madrid, Spain (2012)
  • Super-resolution microscopy and its biological applications M. Lakadamyali Karolinska Institute, Stockholm, Sweden (2012)
  • Probing cargo transport with correlated live cell and super-resolution microscopy M. Lakadamyali European Microscopy Congress, Manchester, UK (2012)
  • Probing cargo transport with correlated live cell and super-resolution microscopy M. Lakadamyali Swiss Single Molecule Localization Microscopy Symposium, Lausanne, Switzerland (2012)
  • Super-resolution imaging and its applications to biology M. Lakadamyali IMPPC, Barcelona, Spain (2012)
  • Capturing life’s processes with light M. Lakadamyali TEDxBarcelona Science, Barcelona, Spain (2011)
  • Super-resolution microscopy M. Lakadamyali Imaging Cellular Structure across Scales, Working Group, American Society for Cell Biology Meeting, Denver, CO, USA (2011)
  • Capturing life’s processes with light M. Lakadamyali TEDxBarcelona Science, Barcelona, Spain (2011)
  • Super-resolution imaging and its applications to biology M. Lakadamyali Workshop in Super-resolution and 4D Confocal Imaging, Umea, Sweden (2011)
  • Super-resolution imaging and its applications to biology M. Lakadamyali From Quantitative Microscopy to Biophysical Models, Practical Corse in Advanced Light Microscopy, Bayreuth, Germany (2011)
  • Imaging neuronal connectivity at high resolution M. Lakadamyali Neural circuit development and plasticity: progress made and promises ahead, Academy Colloquium, Amsterdam, Netherlands (2011)
  • Imaging neuronal connectivity at high resolution M. Lakadamyali Seeing is believing: Imaging the processes of life symposium, Heidelberg, Germany (2011)
  • Mapping neuronal connectivity with stochastic optical reconstruction microscopy: The BrainSTORM project M. Lakadamyali Advance course in live cell imaging, Oslo, Norway (2010)
    Posters
  • Antimicrobial and biofilm preventing functionalised optical surfaces R. Sibilo, I. Mannelli, M. A. Noyan, R. Reigada, P. Mazumder, M. Lakadamyali, V. Pruneri 5th International Conference on Multifunctional, Hybrid and Nanomaterials, Lisbon, Portugal, March 2017
  • Stoichiometry of the human glycine receptor revealed by direct subunit counting N. Durisic, A.G. Godin, C.M. Wever, C. D. Heyes, M. Lakadamyali*, J. A. Dent*, (co-last authors), Gordon Research Conference, Single Molecule Approaches to Biology, West Dover, VT, USA (2012)
  • Correlative live cell and super resolution imaging of intracellular transport S. Balint, N. Brede, A. Sandoval, M. Lakadamyali Biophysical Society Meeting, San Diego, USA (2012)
  • GraspJ: An open source, real-time analysis package for super-resolution imaging N. Brede, M. Lakadamyali Biophysical Society Meeting, San Diego, USA (2012)
    PhD Theses
  • Unravelling 3D cargo transport dynamics at the microtubule network with super-resolution microscopy I. Verdeny Vilanova

Advanced Fluorescence Imaging and Biophysics

Prof. Dr. Melike Lakadamyali