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Single Molecule Biophotonics

Prof. Dr. María García-Parajo

    Activities:

Our research focuses on the development of advanced optical techniques to the study of biological processes at the single molecular level on living cells. We focus on the development and application of different forms of super-resolution microscopy (STED, STORM, NSOM) as well as photonic antennas to reach spatial resolutions around 10nm on intact cells. Fluorescence correlation spectroscopy in ultraconfined volumes, and multi-color single particle tracking are exploited to gain access to dynamic processes down to the microsecond time resolution. Using these combined approaches, we aim at understanding how spatiotemporal compartmentalization of biomolecules inside cells regulates and control cell function. This fundamental question has important implications for health and disease, touching the fields of cell biology and immunology.

    Available Resources:
  • Combined single molecule sensitive confocal/near-field optical microscope (NSOM) fully adapted for biological imaging in physiological conditions.
  • Single-molecule-detection-sensitive scanning confocal microscopy: imaging, polarization, and wavelength sensitive.
  • Dual-color wide-field/TIRF fluorescence microscopy equipped with dual color detection (EM-CCD camera) and environmental chamber for live cell experiments.
  • Multi-parametric single-molecule confocal (Microtime 200) combined with epi-TIRF excitation and dual color detection (EM-CCD camera). Fully adapted for live cell experiments.
  • Lasers: ps-diode-lasers, Ar/Kr+, several HeNe, etc.
  • State-of-art biochemical and cell culture Lab.
    Funding:
  • ICREA – Institució Catalana de Recerca i Estudis Avançats
  • AGAUR – Agència de Gestió d’Ajuts Universitaris i de Recerca. 2009 SGR 597
  • MICINN – Plan Nacional MAT2011-22887: IMMU-NANO-FORCE
  • La marató de TV3 – Development of nanomedicines for enzymatic replacement therapy in Fabry disease.
  • FP7-ICT-2011-7 - NANO-VISTA – Advanced photonic antenna tools for biosensing and cellular nanoimaging (coordinator)
    Collaborations:
  • Prof. Carl G. Figdor, Nijmegen Centre for Molecular Life Sciences (NCMLS), Nijmegen (The Netherlands).
  • Dr. Alessandra Cambi, Nijmegen Centre for Molecular Life Sciences (NCMLS), Nijmegen (The Netherlands)
  • Prof. Peter Hinterdorfer, Biophysics Institute, Johannes Kepler University, Linz (Austria).
  • Prof. Vincenzo Cerundolo, Weatherall Institute of Molecular Medicine, University of Oxford (UK).
  • Prof. Jürgen Brugger, École Polytechnique Fédérale de Lausanne (Switzerland).
  • Prof. Hervé Rigneault, Institut Fresnel, Marseille (France).
  • Prof. Niek van Hulst, Institut de Ciències Fotòniques (ICFO), Barcelona (Spain).

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Single Molecule Biophotonics

Prof. Dr. María García-Parajo

    Former Group Members
  • Sonja Buschow, Visiting scientist
  • Bruno Miguel Castro, Postdoctoral researcher
  • Erik Garbacik, Postdoctoral researcher
  • Izabela Katarzyna Piechocka, Postdoctoral researcher
  • Claude Renaut, Postdoctoral researcher
  • Juan Andrés Torreño, PhD student
  • Thomas Sebastiaan van Zanten, Postdoctoral researcher
  • Alberto Sosa, PhD student

Single Molecule Biophotonics

Prof. Dr. María García-Parajo

    Proceedings & Book Chapters
  • Near-field optical nanoscopy of biological membranes T. S. van Zanten, C. Manzo, M. F. Garcia-Parajo Properties and functions of biological membranes investigated by fluorescence methods, Y. Mely, G. Duportail (eds.)
    Springer Series Fluorescence, Springer-Verlag Berlin Heidelberg (2012)
  • Superresolution near-field optical microscopy T. S. van Zanten, M. F. Garcia-Parajo Comprehensive Biophysics, P. Schwille (Ed.) Elsevier (2012)
  • The role of nanophotonics in regenerative medicine M. F. Garcia-Parajo Nanotechnology in regenerative medicine, M. Navarro, J. A. Planell (Eds.) Humana Press (2011)
    Invited Talks
  • Cell surface nanoscopy and spectroscopy using single molecule optical nano-tools M. F. García-Parajo Department of Physics and Astronomy, University of Sheffield, UK, 05 June 2013
  • Effect of mechanical forces on the spatiotemporal organization of the integrin receptor LFA-1 M. F. García-Parajo International conference in Mechanical manipulations and responses at the scale of the cell and beyond, National Centre for Biological Sciences, Bangalore, India, 19 April 2013
  • Optical nano-tools and mechanical manipulation for subcellular studies M. F. García-Parajo International workshop in Mechanical manipulations and responses at the scale of the cell and beyond, National Centre for Biological Sciences, Bangalore, India, 17 April 2013
  • From nano to meso-scale organization of DC-SIGN: a relationship between large scale ordering and dynamics M. F. García-Parajo University of North Caroline, Chapel Hill, USA, 07 February 2013
  • Superresolution nanoscopy for cell membrane studies at the single molecule level M. F. García-Parajo 1st Congress of the Spanish network of advanced optical microscopy, Barcelona, Spain, 21 November 2012
  • Cell surface nanoscopy and spectroscopy using single molecule optical nano-tools M. F. García-Parajo MediNano-5, ICFO, Castelledefels, Spain, 05-06 November 2012
  • Biophysics of Leukocyte adhesion: Nanoscale dynamics of the integrin receptor LFA-1 M. F. García-Parajo Severo Ochoa Seminars, CNIO, Madrid, Spain, 24 September 2012
  • Total internal reflection microscopy”, FEBS 2012 course in Microspectroscopy: visualization of protein dynamics in living cells M. F. Garcia-Parajo FEBS 2012, Nijmegen, the Netherlands, 05-07 September, 2012
  • Single molecule nanophotonics and its applications in Biology M. F. Garcia-Parajo Summer Lecture ICFO, Castelldefels, Spain, 25 July, 2012
  • Cell surface nanoscopy using single molecule optical nanotools M. F. Garcia-Parajo Conference in Optics within Life Sciences, Genoa, Italy, 04-07 July, 2012 Plenary Lecture
  • Lipids and integrin nanodomains in leukocyte cell membranes M. F. Garcia-Parajo CNIC seminar, Madrid, Spain, 23 April, 2012
  • Single molecule nanophotonics and its applications in Biology M. F. Garcia-Parajo VII jornadas cientificas IUMA, Alicante, Spain, 19 January, 2012
  • Single molecule nanophotonics and its applications in Biology M. F. Garcia-Parajo XIV congreso de la Sociedad espanola de biologia celular, Malaga, Spain, 12-15 December, 2011
  • Far-field nanoscopy M. F. Garcia-Parajo Nanosciences for life, 
Santarém, Portugal, 17-19 November, 2011
  • Near-field nanoscopy M. F. Garcia-Parajo Nanosciences for life, 
Santarém, Portugal, 17-19 November, 2011
  • Single molecule imaging of cell surfaces using near-field nanoscopy M. F. Garcia-Parajo BCN-NANO, Barcelona, Spain, 20 September, 2011
  • Compositional connectivity of cell membranes: nanoimaging and nanospectroscopy M. F. Garcia-Parajo GEN-AU Summer School, Litschau, Austria, 30 Aug-2 September, 2011
  • Single molecule nanophotonics and its applications in Biology M. F. Garcia-Parajo ICFO summer courses, Castelldefels, Spain, 27 July, 2011
  • An introduction to biological membranes M. F. Garcia-Parajo ICFO-L4H tutorial to Biology, Castelldefels, Spain, 12 July, 2011
  • Nanoimaging and nanospectroscopy of living cells using near-field approaches M. F. Garcia-Parajo ELMI meeting, Alexandropoulos, Greece, 07-11 June, 2011
  • BioNanoPhotonics: using light to explore biological cells at the nano level M. F. Garcia-Parajo 2nd Annual HECBC Lecture, Penn State Berks College, Reading, USA, 30 March, 2011 Plenary Lecture
  • Nanotechnology at a glance M. F. Garcia-Parajo Future trends in Nanotechnology as part of the Keystone Innovation zone, Penn State Berks College, Reading, USA, 30 March, 2011 Keynote speaker
  • Superresolution light nanoscopy M. F. Garcia-Parajo First meeting of the Spanish Society for Advanced Microscopy, Barcelona, Spain, 2 March, 2011
  • An optical view of cell membrane compartmentalization and functional implications M. F. Garcia-Parajo International Symposium on "Lipid and Protein Nanoislands: Organization, Dynamics and Signalling", Nijmegen, Netherlands, 27-28 January, 2011
    PhD Theses
  • Insights on the spatiotemporal organization of integrins and their ligands using quantitative biophysical tools A. Sosa Costa
  • Membrane protein nanoclustering as a functional unit of immune Cells - from nanoscopy to single molecule dynamics- J. A. Torreño Piña

Single Molecule Biophotonics

Prof. Dr. María García-Parajo