Multimodal Digital Scanned Light Sheet Microscopy (mDSLM)
The multimodal SPIM/DSLM system is a custom-made microscope designed to explore large microscopic 3D samples such as organoids or small embryos.
It is highly versatile for addressing diverse biological questions thanks to its modular fashion. This modular microscope allows keeping a controlled physiological environment that enables recording the fast biological dynamics in 3D.
Excitation Modalities
Linear and non-linear fluorescence
Techniques
TPEF, Multimodal Digital Scanned Light Sheet Microscopy, Confocal Line Scan.
Microscope objectives
- for excitation: 10X, 0.3 NA and 10X, 0.45 NA
- for collection (water inmmersion): 20X, 0.5 NA and 25X, 1.05 NA
Excitation wavelengths
- Diode lasers 405nm, 488nm, 638nm
- DPSS laser 532nm, 651nm
- Femtosecond laser 750-850nm, 1030nm, 1550nm
Detectors
Hamamatsu Orca Flash 4