Leica TCS SP8 STED 3X-FALCON
The Leica TCS SP8 STED 3X-FALCON is based on the TCS SP8 confocal microscope. The STED 3X module allows 3D super-resolution capabilities in 3 optical bands due to its 3 depletion lasers. The FALCON (FAst Lifetime CONtrast) is a fluorescence lifetime imaging microscopy (FLIM) platform fully integrated in the confocal microscope that can deliver video-rate FLIM with pixel-by-pixel quantification.
The single molecule detector (SMD HyD) provide a high detection efficiency. Resonant scanners provide fast scanning capabilities up to video-rate at 512×512 pixels. The system includes a white light laser source as well as a 405 nm semiconductor laser. Live imaging is possible due to controlled environmental conditions.
Techniques
STED 3D and 3 colours, Confocal, FLIM, Full-hyper spectral (6D) laser scanning confocal.
Microscope objectives
10x, 63x and 100x
Excitation wavelengths
- white light laser: 470-670 nm
- Semiconductor: 405 nm
- 8 simultaneous excitation laser lines
- STED lasers: 592nm, 660nm, 775nm
Detectors
- 2 Backwards HyD’s
- 1 Forward PMT
- 2 Backwards PMT’s
Scanning mirrors
- Galvanometric
- Resonant