Light Seminars
February 18, 2015
L4H Seminar RAINER HEINTZMANN 'Structured Illumination and the Analysis of Single Molecules in Cells'
L4H Seminar RAINER HEINTZMANN 'Structured Illumination and the Analysis of Single Molecules in Cells'
NER HEINTZMANN
Leibniz Institute of Photonic Technology & Frie
Wednesday, February 18, 2015, 12:00. Seminar Room
RAINER HEINTZMANN
Leibniz Institute of Photonic Technology & Friedrich-Schiller-Universität Jena
King's College London
RAINER HEINTZMANN
Leibniz Institute of Photonic Technology & Friedrich-Schiller-Universität Jena
King's College London
In the past decade revolutionary advances have been made in the field of microscopy imaging, some of which have been honored by the Nobel prize in Chemistry 2014. One high-resolution method is based on transforming conventionally unresolvable details into measurable patterns with the help of an effect most people have already personally experienced: the Moiré effect. If two fine periodic patterns overlap, coarse patterns emerge. This is typically seen on a finely weaved curtain folding back onto itself. Another example is fast moving coarse patterns on both fences of a bridge above a motorway, when approaching it with the car. The microscopy method of structured illumination utilizes this effect by projecting a fine grating onto the sample and imaging the resulting coarser Moiré patterns containing the information about invisibly fine sample detail. With the help of computer reconstruction based on several such Moiré images, a high-resolution image of the sample can then be assembled.
Another way to obtain a high-resolution map of the sample is to utilize the blinking behavior inherent in most molecules, used to stain the sample. Recent methodological advances (Cox et al., Nature Methods 9, 195-200, 2012) enable us to create pointillist high-resolution maps of molecular locations in a living biological sample, even if in each of the required many individual images, these molecules are not individually discernible. Examples will be shown as a film of a cell at 30 millionths of millimeter resolution and 6 seconds between the individual movie frames.
Wednesday, February 18, 2015, 12:00. Seminar Room
Hosted by Dr. Pablo Loza
Another way to obtain a high-resolution map of the sample is to utilize the blinking behavior inherent in most molecules, used to stain the sample. Recent methodological advances (Cox et al., Nature Methods 9, 195-200, 2012) enable us to create pointillist high-resolution maps of molecular locations in a living biological sample, even if in each of the required many individual images, these molecules are not individually discernible. Examples will be shown as a film of a cell at 30 millionths of millimeter resolution and 6 seconds between the individual movie frames.
Wednesday, February 18, 2015, 12:00. Seminar Room
Hosted by Dr. Pablo Loza
Light Seminars
February 18, 2015
L4H Seminar RAINER HEINTZMANN 'Structured Illumination and the Analysis of Single Molecules in Cells'
L4H Seminar RAINER HEINTZMANN 'Structured Illumination and the Analysis of Single Molecules in Cells'
NER HEINTZMANN
Leibniz Institute of Photonic Technology & Frie
Wednesday, February 18, 2015, 12:00. Seminar Room
RAINER HEINTZMANN
Leibniz Institute of Photonic Technology & Friedrich-Schiller-Universität Jena
King's College London
RAINER HEINTZMANN
Leibniz Institute of Photonic Technology & Friedrich-Schiller-Universität Jena
King's College London
In the past decade revolutionary advances have been made in the field of microscopy imaging, some of which have been honored by the Nobel prize in Chemistry 2014. One high-resolution method is based on transforming conventionally unresolvable details into measurable patterns with the help of an effect most people have already personally experienced: the Moiré effect. If two fine periodic patterns overlap, coarse patterns emerge. This is typically seen on a finely weaved curtain folding back onto itself. Another example is fast moving coarse patterns on both fences of a bridge above a motorway, when approaching it with the car. The microscopy method of structured illumination utilizes this effect by projecting a fine grating onto the sample and imaging the resulting coarser Moiré patterns containing the information about invisibly fine sample detail. With the help of computer reconstruction based on several such Moiré images, a high-resolution image of the sample can then be assembled.
Another way to obtain a high-resolution map of the sample is to utilize the blinking behavior inherent in most molecules, used to stain the sample. Recent methodological advances (Cox et al., Nature Methods 9, 195-200, 2012) enable us to create pointillist high-resolution maps of molecular locations in a living biological sample, even if in each of the required many individual images, these molecules are not individually discernible. Examples will be shown as a film of a cell at 30 millionths of millimeter resolution and 6 seconds between the individual movie frames.
Wednesday, February 18, 2015, 12:00. Seminar Room
Hosted by Dr. Pablo Loza
Another way to obtain a high-resolution map of the sample is to utilize the blinking behavior inherent in most molecules, used to stain the sample. Recent methodological advances (Cox et al., Nature Methods 9, 195-200, 2012) enable us to create pointillist high-resolution maps of molecular locations in a living biological sample, even if in each of the required many individual images, these molecules are not individually discernible. Examples will be shown as a film of a cell at 30 millionths of millimeter resolution and 6 seconds between the individual movie frames.
Wednesday, February 18, 2015, 12:00. Seminar Room
Hosted by Dr. Pablo Loza
All Insight Seminars
Light Seminars
November 23, 2015
L4H Seminar LUIS DE LECEA 'Optogenetic Control of Arousal'
Light Seminars
November 18, 2015
L4H Seminar JAN BRUGUES 'Elucidating the Physical Basis of Spindle Self-Organisation via Microscopy'
Light Seminars
October 28, 2015
L4H Seminar PETER ZIJLSTRA 'Single-Particle And Single-Molecule Plasmonic Sensing'
Light Seminars
October 21, 2015
L4H Seminar LUKAS KAPITEIN ‘Navigating the Neuronal Cytoskeleton: Novel Tools to Dissect and Direct Intracellular Transport’
Light Seminars
July 15, 2015
L4H Seminar ELLEN GRANT 'Potential Applications of Optical Imaging in Neonatology'
Light Seminars
May 20, 2015
L4H Seminar ELISABET ROMERO 'The Quantum Design of Solar-Energy Conversion in Photosynthesis: From Understanding to Engineering'
Light Seminars
May 6, 2015
L4H Seminar LOTHAR SCHERMELLEH '3D super-resolution imaging of functional chromatin topology'
Light Seminars
April 22, 2015
L4H Seminar SEBASTIAN MAERKL 'Microfluidic Large Scale Integration and its Application to Human Health'
Light Seminars
March 25, 2015
L4H Seminar HARSHAD VISHWASRAO 'Imaging the Molecular Interaction Network of the Actin Cytoskeleton'
Light Seminars
January 21, 2015
L4H Seminar PIERRE MAHOU 'Study of Neurodegenerative Diseases with STED Microscopy: From the Technique to the Application'