In the fluorescence microscopy field, much interest has focused on new super-resolution techniques (collectively known as PALM/STORM, STED, SIM and others) that demonstrated to bypass the diffraction limit and provide a spatial resolution reaching a near-molecular level. With these techniques, it has become possible to image cellular structures in far greater detail than ever before. Single-molecule based methods such as photoactivation-localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM) and directSTORM (dSTORM) employ photoswitchable fluorophores and single-molecule localization to generate a super-resolution image. The important next step is to obtain reliable quantitative information from super-resolution images of cellular structures. Single-molecule super-resolution techniques can be applied to study the aggregation of membrane proteins and the nano-structural organization of HIV-1 envelope protein in intact cells and single viruses. Furthermore, single-molecule counting of nucleosome-associated proteins and transcription-associated proteins will be introduced.


Wednesday, September 4, 2013, 12:00. Seminar Room

Hosted by Prof. María García-Parajo