Seminars
February 1, 2008
JEAN-MARC FOURNIER 'Optical binding and multiple trapping'
JEAN-MARC FOURNIER 'Optical binding and multiple trapping'
Prof. JEAN-MARC FOURNIER
Imaging and Applied Optics Institute
Seminar, February 1st,
12:00. Seminar Room
Prof. JEAN-MARC FOURNIER
Imaging and Applied Optics Institute
École Polytechnique Fédérale de Lausanne
SWITZERLAND
12:00. Seminar Room
Prof. JEAN-MARC FOURNIER
Imaging and Applied Optics Institute
École Polytechnique Fédérale de Lausanne
SWITZERLAND
Combinations of microfluidics with multiple optical tweezers, capable of creating free-floating arrays of objects, are used for performing controlled, parallel and simultaneous (bio)chemical reactions. Such objects might be beads, cells, cell fragments, vesicles etc. We show how they can be physically and chemically addressed, manipulated and detected. The approach using vesicles will allow the ultimate downscaling of bioanalytics to the nanometer and attoliter range.
Several methodologies are considered, from trapping in interference patterns to building arrays of optical traps with micro-optical elements.
Arrangements of optical traps easily built from constructive and destructive interference of coherent light waves are first presented. For example, interference of several identical Gaussian light beams distributed with n-fold symmetries lead to periodic or aperiodic arrays of traps. Such superimposition of coherent complex amplitude distributions can lead to atypical intensity landscapes presenting sharp gradients and offering high potential for strong optical trapping. For example, a set of traps made with multiple beam interference using an interferometer of the Tolansky-Fizeau type is particularly efficient.
Multiple optical tweezers generated with arrays of high-NA micro-optical components (parabolic micro-mirrors) allow multiple 3D optical trapping with no need for objective lenses. Moreover, arrays of such micro-mirrors provide a highly scalable approach for generating a very large number of optical traps, overcoming the field-of-view limitation of conventional tweezers based on microscope objectives, and may straightforwardly be integrated in microfluidic devices.
The interaction between dielectric particles bathed in an intense electromagnetic field leads to mutual trapping and therefore to self-organization through optical binding. The complexity of momentum exchange between a light field and matter starts to be deciphered through rigorous electromagnetic calculations; meanwhile, several experiments investigate the many-body problem through observations and measurement of optically bound matter.
Seminar, 1st of February, 12:00h. Conference Room
Hosted by Prof. Dmitri Petrov
Several methodologies are considered, from trapping in interference patterns to building arrays of optical traps with micro-optical elements.
Arrangements of optical traps easily built from constructive and destructive interference of coherent light waves are first presented. For example, interference of several identical Gaussian light beams distributed with n-fold symmetries lead to periodic or aperiodic arrays of traps. Such superimposition of coherent complex amplitude distributions can lead to atypical intensity landscapes presenting sharp gradients and offering high potential for strong optical trapping. For example, a set of traps made with multiple beam interference using an interferometer of the Tolansky-Fizeau type is particularly efficient.
Multiple optical tweezers generated with arrays of high-NA micro-optical components (parabolic micro-mirrors) allow multiple 3D optical trapping with no need for objective lenses. Moreover, arrays of such micro-mirrors provide a highly scalable approach for generating a very large number of optical traps, overcoming the field-of-view limitation of conventional tweezers based on microscope objectives, and may straightforwardly be integrated in microfluidic devices.
The interaction between dielectric particles bathed in an intense electromagnetic field leads to mutual trapping and therefore to self-organization through optical binding. The complexity of momentum exchange between a light field and matter starts to be deciphered through rigorous electromagnetic calculations; meanwhile, several experiments investigate the many-body problem through observations and measurement of optically bound matter.
Seminar, 1st of February, 12:00h. Conference Room
Hosted by Prof. Dmitri Petrov
Seminars
February 1, 2008
JEAN-MARC FOURNIER 'Optical binding and multiple trapping'
JEAN-MARC FOURNIER 'Optical binding and multiple trapping'
Prof. JEAN-MARC FOURNIER
Imaging and Applied Optics Institute
Seminar, February 1st,
12:00. Seminar Room
Prof. JEAN-MARC FOURNIER
Imaging and Applied Optics Institute
École Polytechnique Fédérale de Lausanne
SWITZERLAND
12:00. Seminar Room
Prof. JEAN-MARC FOURNIER
Imaging and Applied Optics Institute
École Polytechnique Fédérale de Lausanne
SWITZERLAND
Combinations of microfluidics with multiple optical tweezers, capable of creating free-floating arrays of objects, are used for performing controlled, parallel and simultaneous (bio)chemical reactions. Such objects might be beads, cells, cell fragments, vesicles etc. We show how they can be physically and chemically addressed, manipulated and detected. The approach using vesicles will allow the ultimate downscaling of bioanalytics to the nanometer and attoliter range.
Several methodologies are considered, from trapping in interference patterns to building arrays of optical traps with micro-optical elements.
Arrangements of optical traps easily built from constructive and destructive interference of coherent light waves are first presented. For example, interference of several identical Gaussian light beams distributed with n-fold symmetries lead to periodic or aperiodic arrays of traps. Such superimposition of coherent complex amplitude distributions can lead to atypical intensity landscapes presenting sharp gradients and offering high potential for strong optical trapping. For example, a set of traps made with multiple beam interference using an interferometer of the Tolansky-Fizeau type is particularly efficient.
Multiple optical tweezers generated with arrays of high-NA micro-optical components (parabolic micro-mirrors) allow multiple 3D optical trapping with no need for objective lenses. Moreover, arrays of such micro-mirrors provide a highly scalable approach for generating a very large number of optical traps, overcoming the field-of-view limitation of conventional tweezers based on microscope objectives, and may straightforwardly be integrated in microfluidic devices.
The interaction between dielectric particles bathed in an intense electromagnetic field leads to mutual trapping and therefore to self-organization through optical binding. The complexity of momentum exchange between a light field and matter starts to be deciphered through rigorous electromagnetic calculations; meanwhile, several experiments investigate the many-body problem through observations and measurement of optically bound matter.
Seminar, 1st of February, 12:00h. Conference Room
Hosted by Prof. Dmitri Petrov
Several methodologies are considered, from trapping in interference patterns to building arrays of optical traps with micro-optical elements.
Arrangements of optical traps easily built from constructive and destructive interference of coherent light waves are first presented. For example, interference of several identical Gaussian light beams distributed with n-fold symmetries lead to periodic or aperiodic arrays of traps. Such superimposition of coherent complex amplitude distributions can lead to atypical intensity landscapes presenting sharp gradients and offering high potential for strong optical trapping. For example, a set of traps made with multiple beam interference using an interferometer of the Tolansky-Fizeau type is particularly efficient.
Multiple optical tweezers generated with arrays of high-NA micro-optical components (parabolic micro-mirrors) allow multiple 3D optical trapping with no need for objective lenses. Moreover, arrays of such micro-mirrors provide a highly scalable approach for generating a very large number of optical traps, overcoming the field-of-view limitation of conventional tweezers based on microscope objectives, and may straightforwardly be integrated in microfluidic devices.
The interaction between dielectric particles bathed in an intense electromagnetic field leads to mutual trapping and therefore to self-organization through optical binding. The complexity of momentum exchange between a light field and matter starts to be deciphered through rigorous electromagnetic calculations; meanwhile, several experiments investigate the many-body problem through observations and measurement of optically bound matter.
Seminar, 1st of February, 12:00h. Conference Room
Hosted by Prof. Dmitri Petrov