Seminars
June 8, 2006
IVAN AMAT ROLDÁN, 'Detecting nonmelanoma skin cancers using multi-modal confocal microscopy'
IVAN AMAT ROLDÁN, 'Detecting nonmelanoma skin cancers using multi-modal confocal microscopy'
IVAN AMAT ROLDÁN
Seminar, 8th of June, 18:00h. Conference Room
IVAN AMAT ROLDÁN
Ultrafast imaging and nonlinear microscopy
ICFO-Institute of Photonic Sciences
SPAIN
IVAN AMAT ROLDÁN
Ultrafast imaging and nonlinear microscopy
ICFO-Institute of Photonic Sciences
SPAIN
Background and Significance: Early detection of neoplasm is imperative for successful cancer treatments. The goal of this study was to establish the feasibility of using dye-enhanced multi-modal confocal microscopy as a tool for detecting different types of nonmelanoma skin cancers.
Materials and Methods: Freshly excised thick skin samples were used for the experiments. The specimens were rapidly stained in aqueous solutions of either toluidine blue or methylene blue and imaged using multi-spectral confocal reflectance and fluorescence microscope. Reflectance images were acquired at the wavelengths of 633nm, 656 nm, and 830nm. Fluorescence was excited at 633 nm and 656 nm. Fluorescence emission was registered in the range between 670 nm and 690 nm. The resulting images were compared to the corresponding en face frozen H&E sections.
Results and Conclusion: The results of the study indicate that dye-enhanced multi-spectral reflectance and fluorescence confocal images provide complementary information on tissue morphology and dye uptake. Confocal images of stained skin closely resemble corresponding H&E sections, enabling interpretation of confocal images and cancer detection in a same manner as histopathology.
Seminar, 8th of June, 18:00h. Conference Room
Materials and Methods: Freshly excised thick skin samples were used for the experiments. The specimens were rapidly stained in aqueous solutions of either toluidine blue or methylene blue and imaged using multi-spectral confocal reflectance and fluorescence microscope. Reflectance images were acquired at the wavelengths of 633nm, 656 nm, and 830nm. Fluorescence was excited at 633 nm and 656 nm. Fluorescence emission was registered in the range between 670 nm and 690 nm. The resulting images were compared to the corresponding en face frozen H&E sections.
Results and Conclusion: The results of the study indicate that dye-enhanced multi-spectral reflectance and fluorescence confocal images provide complementary information on tissue morphology and dye uptake. Confocal images of stained skin closely resemble corresponding H&E sections, enabling interpretation of confocal images and cancer detection in a same manner as histopathology.
Seminar, 8th of June, 18:00h. Conference Room
Seminars
June 8, 2006
IVAN AMAT ROLDÁN, 'Detecting nonmelanoma skin cancers using multi-modal confocal microscopy'
IVAN AMAT ROLDÁN, 'Detecting nonmelanoma skin cancers using multi-modal confocal microscopy'
IVAN AMAT ROLDÁN
Seminar, 8th of June, 18:00h. Conference Room
IVAN AMAT ROLDÁN
Ultrafast imaging and nonlinear microscopy
ICFO-Institute of Photonic Sciences
SPAIN
IVAN AMAT ROLDÁN
Ultrafast imaging and nonlinear microscopy
ICFO-Institute of Photonic Sciences
SPAIN
Background and Significance: Early detection of neoplasm is imperative for successful cancer treatments. The goal of this study was to establish the feasibility of using dye-enhanced multi-modal confocal microscopy as a tool for detecting different types of nonmelanoma skin cancers.
Materials and Methods: Freshly excised thick skin samples were used for the experiments. The specimens were rapidly stained in aqueous solutions of either toluidine blue or methylene blue and imaged using multi-spectral confocal reflectance and fluorescence microscope. Reflectance images were acquired at the wavelengths of 633nm, 656 nm, and 830nm. Fluorescence was excited at 633 nm and 656 nm. Fluorescence emission was registered in the range between 670 nm and 690 nm. The resulting images were compared to the corresponding en face frozen H&E sections.
Results and Conclusion: The results of the study indicate that dye-enhanced multi-spectral reflectance and fluorescence confocal images provide complementary information on tissue morphology and dye uptake. Confocal images of stained skin closely resemble corresponding H&E sections, enabling interpretation of confocal images and cancer detection in a same manner as histopathology.
Seminar, 8th of June, 18:00h. Conference Room
Materials and Methods: Freshly excised thick skin samples were used for the experiments. The specimens were rapidly stained in aqueous solutions of either toluidine blue or methylene blue and imaged using multi-spectral confocal reflectance and fluorescence microscope. Reflectance images were acquired at the wavelengths of 633nm, 656 nm, and 830nm. Fluorescence was excited at 633 nm and 656 nm. Fluorescence emission was registered in the range between 670 nm and 690 nm. The resulting images were compared to the corresponding en face frozen H&E sections.
Results and Conclusion: The results of the study indicate that dye-enhanced multi-spectral reflectance and fluorescence confocal images provide complementary information on tissue morphology and dye uptake. Confocal images of stained skin closely resemble corresponding H&E sections, enabling interpretation of confocal images and cancer detection in a same manner as histopathology.
Seminar, 8th of June, 18:00h. Conference Room