All day
Place: ICFO Auditorium
Pablo Loza (ICFO)
"Fast volumetric imaging in neuronal organoids and model organisms"
Abstract:
In Light sheet fluorescence microscopy (LSFM), a sheet of excitation light is produced onto the sample plane. The generated fluorescence is then collected using a microscope objective placed orthogonal to the excitation light sheet plane. LSFM allows for a highly efficient excitation and collection of the generated signal. Altogether, such scheme minimise light dose onto the sample and results in a decreased photobleaching, reducing thus phototoxic effects.
LSFM has been put forward as an interesting candidate for fast volumetric imaging of biological samples. Here, I will present our efforts for achieving fast 3D imaging for high through put applications based a fluidic system based on the use of a FEP tube and a syringe pump. During the second part, I will present a LSFM microscope for fast volumetric imaging based on the use of an electrically tunable lens (ETL). This system is used to image the spontaneous Ca2+activity, as reported by GCaMP fluorescence.
Bio:
Pablo Loza-Alvarez (PhD). Dr. Loza is the head of the “Super Resolution Light Microscopy and Nanoscopy” lab at ICFO. Dr. Loza-Alvarez has a strong experience in microscopy and, by introducing novel photonics tools, has developed a number of novel imaging techniques. These have been used for a wide variety of applications ranging from the imaging of large model organisms (mesoscopic level) to the visualization of subcellular components (super resolution). He has directed 8 PhD students, co-authored over 140+ publications in international journals (h=42, Google Scholar), has written 7 patents and he is regularly invited for seminars at different conferences, schools and colloquia. Dr Loza-Alvarez, has experience in leading European and national projects (public or private funding) and has been active in several Networks of Excellence and doctoral training networks. Dr. Loza-Alvarez’ group participates in two Euro-BioImaging Nodes: Super Resolution Node Barcelona and Barcelona Mesoscopic Imaging Node.
All day
Place: ICFO Auditorium
Pablo Loza (ICFO)
"Fast volumetric imaging in neuronal organoids and model organisms"
Abstract:
In Light sheet fluorescence microscopy (LSFM), a sheet of excitation light is produced onto the sample plane. The generated fluorescence is then collected using a microscope objective placed orthogonal to the excitation light sheet plane. LSFM allows for a highly efficient excitation and collection of the generated signal. Altogether, such scheme minimise light dose onto the sample and results in a decreased photobleaching, reducing thus phototoxic effects.
LSFM has been put forward as an interesting candidate for fast volumetric imaging of biological samples. Here, I will present our efforts for achieving fast 3D imaging for high through put applications based a fluidic system based on the use of a FEP tube and a syringe pump. During the second part, I will present a LSFM microscope for fast volumetric imaging based on the use of an electrically tunable lens (ETL). This system is used to image the spontaneous Ca2+activity, as reported by GCaMP fluorescence.
Bio:
Pablo Loza-Alvarez (PhD). Dr. Loza is the head of the “Super Resolution Light Microscopy and Nanoscopy” lab at ICFO. Dr. Loza-Alvarez has a strong experience in microscopy and, by introducing novel photonics tools, has developed a number of novel imaging techniques. These have been used for a wide variety of applications ranging from the imaging of large model organisms (mesoscopic level) to the visualization of subcellular components (super resolution). He has directed 8 PhD students, co-authored over 140+ publications in international journals (h=42, Google Scholar), has written 7 patents and he is regularly invited for seminars at different conferences, schools and colloquia. Dr Loza-Alvarez, has experience in leading European and national projects (public or private funding) and has been active in several Networks of Excellence and doctoral training networks. Dr. Loza-Alvarez’ group participates in two Euro-BioImaging Nodes: Super Resolution Node Barcelona and Barcelona Mesoscopic Imaging Node.